A selection of sequence from the transcript tab: cDNA view. Sequence and line numbering in the top line corresponds to the transcript, including UTR, highlighted in bright yellow. The second line corresponds to the coding sequence only. Codons in the first two lines are revealed by light yellow highlighting, alternating with no highlighting. The third line shows the amino acid sequence. The “N” at position 26 in the amino acid sequence is shown in red, indicating another amino acid is possible, depending on the nucleotide allele. The “R” above the highlighted nucleotide is the IUPAC code for purine (A or G), and can be clicked to open the variation tab. For color version of this figure go to http://www.currentprotocols.com/protocol/hg0611.