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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: J Neurosci Methods. 2011 Feb 15;197(1):48–55. doi: 10.1016/j.jneumeth.2011.01.029

Figure 3.

Figure 3

Green autofluorescent cells in hippocampus and cortex represent a population of neurons. Green autofluorescent (AF) cells in area CA1 of hippocampus are immunopositive for NeuN (A) and β-tubulin III (B). Single optical slices (5 μm) of this area obtained with confocal microscopy confirm that the autofluorescent and immunofluorescent signals are localized to the same cells for NeuN (C) and β-tubulin III (D). For C and D, colors are separated for each slice with the left panels (green) showing autofluorescence (C1&D1), the middle panels (red) showing the immunolabel (red, C2&D2) and the right panels showing colocalization of the two signals in the same cells (merge, C3&D3). Epifluorescent micrographs of autofluorescent green cells in cortex labeled for NeuN (E) and β-tubulin III (F). Arrows indicate prominently autofluorescent immunopositive cells. Some autofluorescent cells are indicated with arrows and select autofluorescent cellular processes are delineated with small arrowheads. Scale bars: 50 μm.