Fig. 2.

Effect of mutations L467 V and V586I on TSH stimulated cAMP accumulation. TSHR mutants L467V and V586I are shown as examples for mutants with impaired and unaffected TSH stimulation. cAMP concentration as a function of TSH stimulation was determined in HEK 293 cells transiently transfected either with wild-type or mutated TSHR. Measurement was done by RIA as described in the Materials and methods section. Data are given as mean ± SD. Curves are representative for one out of two independent experiments each done in duplicates