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Vaccine challenge assay. Fish from each group were challenged with 103 Y. ruckeri 150 cells at 28 to 30 days after a 0.1-ml injection of 8 μg of heat-inactivated Yrp1 protease (▪), 102 heat-killed cells (▴), or control with PBS (♦). Dead fish were collected daily. These results represent the average of two independent experiments.
