Table 1.
Microarray results for mouse 297-669 miR cluster in fresh and nutrient-depleted media exposed CHO-S cells. Relative fluorescent signals for all detected (homologous) members of this cluster were higher in depleted media exposed CHO-S cells than in fresh media exposed CHO-S cells after 24h. All detected sequences of mature miRs variants such as 5′-end pre-miR derived (marked 5p) and 3′-end pre-miR derived (marked 3p), and minor miRs variants (marked *) are shown.
| Mouse miR ID (mmu-) | Variant | Mature miR sequence | Fresh media relative fluorescent signal | Depleted media relative fluorescent signal | Up-regulation in depleted media (times) | p-value |
|---|---|---|---|---|---|---|
| miR-297a | - | AUGUAUGUGUGCAUGUGCAUGU | 14±4 | 1412±220 | 101±13 | 0.039 |
| miR-466a | 3p | UAUACAUACACGCACACAUAAGA | 8±1 | 203±30 | 25±1 | 0.050 |
| miR-466b | 3-3p | AAUACAUACACGCACACAUAAGA | 5±1 | 209±39 | 42±1 | 0.035 |
| miR-466d | 3p | UGUGUGUGCGUACAUGUACAUG | 10±1 | 648±122 | 65±6 | 0.039 |
| 5p | UAUACAUACACGCACACAUAG | 8±2 | 552±92 | 69±6 | 0.050 | |
| miR-466f | 3p | CAUACACACACACAUACACAC | 19±1 | 5056±105 | 266±8 | 0.009 |
| 5p | UACGUGUGUGUGCAUGUGCAUG | 102±13 | 22200±5982 | 218±31 | 0.036 | |
| miR-466g | - | AUACAGACACAUGCACACACA | 51±9 | 14935±4286 | 293±32 | 0.038 |
| miR-466h | - | UGUGUGCAUGUGCUUGUGUGUA | 29±4 | 13105±2366 | 452±19 | 0.023 |
| miR-467a | minor | AUAUACAUACACACACCUACAC | 44±1 | 9567±2001 | 217±40 | 0.025 |
| miR-467b | minor | AUAUACAUACACACACCAACAC | 72±8 | 14451±2817 | 201±17 | 0.027 |
| miR-467e | minor | AUAUACAUACACACACCUAUAU | 14±2 | 1056±38 | 75±8 | 0.022 |
| miR-669a | - | AGUUGUGUGUGCAUGUUCAUGU | 7±0 | 57±3 | 8±0 | 0.016 |
| miR-669b | - | AGUUUUGUGUGCAUGUGCAUGU | 8±0 | 46±1 | 6±0 | 0.003 |
| miR-669c | - | AUAGUUGUGUGUGGAUGUGUGU | 23±4 | 2870±299 | 125±9 | 0.023 |
| miR-669d | - | ACUUGUGUGUGCAUGUAUAUGU | 7±0 | 1511±463 | 216±66 | 0.038 |
| miR-669e | - | UGUCUUGUGUGUGCAUGUUCAU | 12±0 | 4090±971 | 341±81 | 0.027 |
| miR-669f | - | CAUAUACAUACACACACACGUAU | 44±1 | 13444±4267 | 306±90 | 0.037 |
| miR-669h | 3p | UAUGCAUAUACACACAUGCACA | 8±0 | 326±6 | 41±1 | 0.007 |
| miR-669i | - | UGCAUAUACACACAUGCAUAC | 18±1 | 39±1 | 2±0 | 0.048 |