Table 2.
Refined set of mmu-miR-466h possible targets. Table shows possible binding site(s) of mmu-miR-466h seed region(s) and mRNA 3′-UTRs of the respective target gene. Brief description of the known anti-apoptotic roles for those genes is given
| Mouse gene symbol | mmu-miR-466h binding site(s) in mRNA 3′-UTR (miR-466h binding nucleotides #) | Anti-apoptotic role of targeted gene |
|---|---|---|
| bcl2 | GCACAC (2–7) | Bcl2 is outer mitochondrial membrane protein which suppresses apoptosis either by preventing release of cytochrome C from mitochondria or by binding apoptosis activating factor (Apaf-1) |
| bcl2l2 | GCACAC(2–7) TGCACA(3–8) |
Bcl2l2 (bcl-w) inhibits formation of permeability transition pore and subsequent release of cytochrome C by binding to bax |
| birc6 | GCACA (3–7) | Birc6 (BRUCE, Apollon) can inhibit apoptosome by binding to Caspase-9 and occupying its active-site pocket. It can function as E2 ubiquitin conjugase for Caspase-9 and Smac/Diablo. Also, in response to loss of BIRC6 function p53 activates PIDD/Caspase-2 and bax/bak resulting in mitochondrial apoptosis |
| cflar | TGCACAC(2–8), 3 of TGCACA(3–8) GCACAC (2–7) |
Caspase-8 and FADD-like apoptosis regulator. Acts as an inhibitor of TNFRSF6 mediated apoptosis. It inhibits procaspase-8 cleavage and Caspase-8 activation. |
| dad1 | 2 of TGCACA (3–8) | Defender against cell death1. It is a component of N-oligosaccharyl transferase catalyzing transfer of oligosaccharide from lipid-linked donor to nascent polypeptide chain. Loss of dad1 was reported to trigger apoptosis |
| naip7 | 2 of GCACAC(2–7) | Naip7 (birc1g) Baculoviral iap repeat-containing 1g protein. BIR domains are known to inhibit apoptosis by direct inhibition of the caspase family of proteases |
| smo | TGCACAC (2–8) GCACAC (2–7) |
Part of Hedgehog signaling pathway. Activated smo uninhibits gli-1 transcriptional factor which stimulates up-regulation of bcl2 |
| stat5a | GCACAC (2–7) | Part of Jak-Stat signaling pathway. Stat5a dimers are transcriptional factors for bcl-xL and bcl2 genes |
| tegt | TGCACAC (2–8) | Tegt is a multipass membrane protein. Interacts with bcl2 and bcl-xL and inhibits bax |