FIG. 5.

A scratch test assay shows hypoxia-induced migration of H9-MSCs in culture. H9-MSCs were plated in a 10 cm tissue culture dish and allowed to grow to 70%–80% confluence. A cell scraper was used to remove the cells in a 5 mm strip along a black line marked on the dish. The cells were then incubated in normoxic (5.0% CO₂/ambient O₂) versus hypoxic conditions (5.0% CO₂, 3.0% O₂) for 24 h. After 24 h of incubation, there was little migration in normoxic conditions (A), while in hypoxic conditions, numerous H9-MSCs had migrated over the black line into the area that had been cleared of cells (B). Color images available online at www.liebertonline.com/tea