FIG. 6.
H9-MSCs engraft in the ischemic muscle tissue of a mouse with a surgically induced ischemic hindlimb. The femoral artery of a NOD/SCID MPSVII mouse was ligated to cause the limb to become ischemic. About 5.0×105 H9-MSCs were injected via the tail vein 24 h after induction of the ischemia. The mouse was sacrificed 24 h postinjection and its tissues were harvested. The tissue was frozen and sectioned on a cryostat. The tissue sections were stained for the presence of beta-glucuronidase, which is not present in the mouse tissue. In the ischemic muscle tissue, the H9-MSCs can be observed around a damaged vessel (A, red enzymatic stain). The contralateral, nonischemic muscle showed no signs of engraftment (B). In a tissue section obtained from the same model of hindlimb ischemia induced in a NOD/SCID β-2-microglobulin knockout mouse (C), the human cells were observed in the ischemic muscle using antibody staining specific for human β-2-microglobulin (green). Scale bar is 50 μm.