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. 2003 Dec;69(12):7507–7513. doi: 10.1128/AEM.69.12.7507-7513.2003

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FIG. 3. — SDS-PAGE of samples from the purification of Nocardia GTPCH. Lane 1, crude extract; lane 2, dialyzed ammonium sulfate precipitate; lane 3, pooled fractions from GTP-agarose; lane 4, pooled fractions from DEAE Sepharose; lane 5, pooled fractions from Ultragel AcA 34; lane 6, control with sample buffer; lane 7, molecular mass markers (phosphorylase b [97.4 kDa], serum albumin [66.2 kDa], ovalbumin [45 kDa], carbonic anhydrase [31 kDa], trypsin inhibitor [21.5 kDa], and lysozyme [14.4 kDa]).