TABLE 1.
Oligonucleotide primers for nucleic acid amplification of Ads
| Ad groups or types | Primer | Target genea | PCR | Sequence (5′→3′) | Amplicon size, bp (reference) |
|---|---|---|---|---|---|
| A-F | Hex1 | Hexon | First | TTCCCCATGGCICA(CT)AACAC | 482 (32) |
| Hex2 | First | CCCTGGTA(GT)CC(AG)AT(AG)TTGTA | |||
| Hex1 | Nested | TTCCCCATGGCICA(CT)AACAC | 443 | ||
| Hex3 | Nested | AGGAACCA(AG)TC(CT)TT(AG)GTCAT | |||
| 2/5 | AdC-E1AF | E1A | First | CCACCTACCCTTCACGAACT | 260 |
| AdC-E1AR | First | CTCGTGGCAGGTAAGATCG | |||
| 40/41 | AdF-E1AF | E1A | First | GGGAACTGGGATGACAT | 280 |
| AdF-E1AR | First | CCSTCTTCATAGCATTTC |
a
The target nucleic acids are both DNA and mRNA.