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Sensitivities as lower detection limits of infectious Ads by conventional and nested PCR assays for different Ad DNA targets
| Ad type | Sensitivity (IU)
|
||
|---|---|---|---|
| PCR with E1A primersa | Hexon primersb
|
||
| PCR | Nested PCR | ||
| Ad2 | 1 | 10 | 0.1 |
| Ad41 | 0.01 | 1 | 0.01 |
PCR with the AdC-E1AF-AdC-E1AR primer pair for Ad2 and the AdF-E1AF-AdF-E1AR primer pair for Ad41.
PCR with the Hex1-Hex2 primer pair and nested PCR with the Hex1-Hex3 primer pair.
