TABLE 3.
Results of culture, PCR, and mRNA RT-PCR of Ad2 disinfected by different Ct doses of free chlorine
| Method | Result obtained with free-chlorine dose as Ct (mg · min/liter)a of:
|
|||
|---|---|---|---|---|
| 0 | 1 | 30 | 100 | |
| CPEb | + | − | − | − |
| DNA detection (PCR) | + | + | + | + |
| Increased viral DNA during culture (PCR) | + | − | − | − |
| mRNA detectionc (RT-PCR) | + | − | − | − |
a
Ct dose = concentration (milligrams per liter) × contact time (minutes) in phosphate buffer (pH = 7.2) at 4°C. Approximately 105 IU of Ad2 was exposed to free chlorine (1 mg/liter) for a predetermined time and then used to inoculate A549 cells.
b
CPE on A549 cells after 7 days.
c
mRNA was extracted from infected A549 cells at 5 to 7 days p.i. and subjected to RT-PCR with the AdC-E1AF-AdC-E1AR primer pair.