TABLE 5.

Results of culture, PCR, and mRNA RT-PCR of Ad2 disinfected by different doses of 254-nm UV radiation

Method	Result obtained with UV dose (mJ/cm2)a of:
	0	50	100	250
CPEb	+	+	+	−
DNA detection (PCR)	+	+	+	+
Increased viral DNA during culture (PCR)	+	+	+	−
mRNA detectionc (RT-PCR)	+	+	+	−

^aDose = UV irradiance (milliwatts per square centimeter) × exposure time (seconds) in PBS (pH = 7.2) at room temperature. Approximately 5 × 10⁵ IU of Ad2 was exposed to 254-nm UV light.

^bCPE on A549 cells after 7 days.

^cmRNA was extracted from infected cells at 5 to 7 days p.i. and subjected to RT-PCR with the AdC-E1AF-AdC-E1AR primer pair.