TABLE 6.
Results of culture, PCR, and mRNA RT-PCR of Ad41 disinfected by different doses of 254-nm UV radiation
| Method | Result obtained with UV dose (mJ/cm2)a of:
|
|||
|---|---|---|---|---|
| 0 | 50 | 100 | 250 | |
| CPEb | − | − | − | − |
| DNA detection (PCR) | + | + | + | + |
| Increased viral DNA during culture (PCR) | + | + | + | − |
| mRNA detectionc (RT-PCR) | + | + | + | − |
a
Dose = UV irradiance (milliwatts per square centimeter) × exposure time (seconds) in PBS (pH = 7.2) at room temperature. Approximately 103 IU of Ad41 was exposed to 254-nm UV light.
b
CPE on Graham293 cells after 7 days.
c
mRNA was extracted from infected G293 cells at 7 days p.i. and subjected to RT-PCR with the Ad1-Ad2 primer pair.