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. Author manuscript; available in PMC: 2011 May 23.
Published in final edited form as: Science. 2010 Nov 12;330(6006):985–989. doi: 10.1126/science.1196554

Fig. 2.

Fig. 2

(A to L) GPR124 functions cell-autonomously in endothelium. Expression of Glut1 is lost in GPR124−/− CNS endothelium, with compensatory Glut1 upregulation in neuroepithelium, [(A) to (D)], E12.5. Glut1 is strongly down-regulated in FACS-isolated, GPR124−/− telencephalic endothelium. qPCR, n=9, E12.5 [(E)]. Selective deletion of GPR124 in FACS-isolated forebrain CD31+ endothelium but not PDGFRβ+ pericytes. qPCR, n=6, E 12.5 [(F)]. E12.5 GPR124flox/−; PDGFB-iCre embryos exhibit forebrain hemorrhaging, formation of PNVP--associated glomeruloid vascular malformations and endothelial Glut1 downregulation [(G) to (L)], recapitulating the global GPR124 deletion phenotype. PNVP, perineural vascular plexus. Error bars are +/− 1 S. D.