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. Author manuscript; available in PMC: 2011 May 23.
Published in final edited form as: J Cell Biochem. 2007 Mar 1;100(4):960–969. doi: 10.1002/jcb.21060

Fig. 1.

Fig. 1

Curcumin inhibits LPS upregulation of atrogin-1/MAFbx gene expression in a dose-dependent manner. Mice receiving curcumin or vehicle for 4 consecutive days were i.p. injected with LPS (1 mg/kg). Gastrocnemius and EDL were collected 12 h after LPS injection. Northern blot analysis was conducted to determine the atrogin-1/MAFbx mRNA isolated from gastrocnemius (panel A). Four mice were used in each group, excluding the control group, which contained three mice. The autoradiograph shown in panel A was quantified via densitometry analysis and normalized to 18S (panel B). Densitometry data (OD) from the Northern analysis of atrogin-1/MAFbx mRNA isolated from EDL are shown in panel C. Three mice were used in each group; however, the control group contained two mice. Panel D shows densitometry data from Northern blot analysis of the effect of curcumin on the basal levels of atrogin-1/MAFbx mRNA in gastrocnemius. Three mice were used in each group. Data were analyzed by ANOVA and *(P < 0.05) or **(P < 0.01) indicates difference from the control group determined by Tukey’s multiple comparison test.