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. Author manuscript; available in PMC: 2011 May 23.
Published in final edited form as: Am J Physiol Cell Physiol. 2006 Dec 6;292(5):C1660–C1671. doi: 10.1152/ajpcell.00486.2006

Fig. 1.

Fig. 1

Differentiating myoblasts increase TNF-α release. Differentiation of C2C12 (A) or mouse primary myoblasts (B) was induced by switching from growth medium (GM) to differentiation medium (DM). At the same time, control cells were changed into fresh GM. At 0, 3, 10, 24, and 48 h, the media were collected and concentrated. TNF-α concentration was determined by ELISA. Two independent experiments were carried out for each type of myoblasts. Data within the same treatment were analyzed with ANOVA, and a difference is indicated next to the curve. The Fisher’s least-significant difference (LSD) multiple-comparison test was performed to compare TNF-α concentration at individual time points with 0 h within the same treatment (difference: *P < 0.01). Student’s t-test was performed to compare TNF-α concentration in DM and GM at each time point (difference: +P < 0.05).