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. Author manuscript; available in PMC: 2011 May 23.
Published in final edited form as: Am J Physiol Cell Physiol. 2006 Dec 6;292(5):C1660–C1671. doi: 10.1152/ajpcell.00486.2006

Fig. 9.

Fig. 9

MKK6bE overexpression in p55−/−p75−/− soleus restores myogenesis. Adeno-virus constructs encoding MKK6bE or GFP cDNA were injected into the hindlimb muscle of 3- to 4-day-old p55−/−p75−/− mice. At 6 wk of age, cardiotoxin was injected into soleus of these mice as well as WT and p55−/−p75−/− mice that were not infected with adenovirus. Soleus was collected 3 days after the cardiotoxin injection. Soleus extracts were analyzed for p38 activation and the expression of differentiation markers (i.e., myogenin, p21, and MHC) via Western blotting. The activation of p38 is expressed as the ratio of the optical density of phosphorylated p38 to that of pan-p38. Expression of the differentiation markers is conveyed as the optical density of the markers normalized to that of β-actin in arbitrary units. Data were analyzed via ANOVA followed by the Fisher’s LSD multiple-comparison test. Differences (P < 0.05) are indicated: a > b > c.