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. 2011 Mar 1;117(17):4580–4589. doi: 10.1182/blood-2010-09-305540

Figure 5.

Figure 5

Quantitative real-time PCR analysis of GATA-1 and PF4 expression. mRNAs extracted from CD34+ cells and from day 14 MKs infected with control (MK + GFP), wild-type (MK + STMN-WT), or nonphosphorylatable mutant (MK + STMN-4A) stathmin-expressing lentiviruses were reverse transcribed and then amplified by quantitative real-time PCR using human GATA-1 (A) and PF4 (B) primers. Each column represents the mean ± SD of 3 independent experiments each performed in duplicate and normalized to GAPDH.