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. 2011 Jan 19;84(6):1190–1197. doi: 10.1095/biolreprod.110.088427

FIG. 6.

FIG. 6.

A) Fluorescence photomicrograph of an F1 embryo produced by a germline chimeric father showing EGFP expression throughout its body indicating that the embryo was produced from sperm derived from the transplanted Tg(vasa:DsRed2-vasa);Tg(bactin:EGFP) double transgenic ovarian germ cells. Because vasa-DsRed expression in the embryo is from maternal RNA, DsRed expression was not observed in the F1 embryos. B, C) The F1 adult fish possessed paired gonads that were of normal size (B, ovary; C, testis) and expressed DsRed (B1 inset and C1 inset, gonads were dissected for fluorescence detection) throughout the tissue. D) The F2 embryos produced by mating heterozygous F1 siblings did express maternal vasa-DsRed in the gonadal region (white arrows) and EGFP expression (black arrows) throughout their bodies in approximately 75% of the F2 embryos. Right bottom corner: schematic drawing of the area where the photo was taken. Bars = 200 μm.