FIG. 1.

Schematic representation of the sakacin TX locus sequenced to date, obtained by using a combination of ligation-anchored PCR and the primer walking strategy. A total of 22 potential ORFs are shown. The arrows indicate ORFs and the proposed directions of transcription. ORFs with deduced functions in production and secretion of active sakacin T and sakacin X are indicated by stx or sak. Sections of the stx locus assumed to be dedicated to production, immunity, regulation, and transport are indicated.