TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source and/or reference |
|---|---|---|
| Strains | ||
| L. sakei 5 | Producer of sakacin T, sakacin X, and sakacin P | Malt (47, 57) |
| L. sakei LMG2313 | Indicator strain sensitive to sakacin T, sakacin X, and sakacin P | LMGa |
| P. pentosaceus LMG2001 | Indicator strain sensitive to sakacin X only | LMG |
| L. sakei Lb790X | Plasmid-free derivative of L. sakei Lb790; Bac− | 6 |
| L. sakei Lb790(pSAK20) | L. sakei Lb790 containing pSAK20, host strain; Bac− Cmr | 5 |
| L. sakei 23K | Plasmid-free L. sakei | 8 |
| E. coli DH5α | φ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 | Promega |
| E. coli EC101 | supE thi Δ(lacproAB) (F′ traD36 proAB lacIq ZΔM15) repA | 35 |
| Plasmids | ||
| pBluescript SK(+) | 3-kb E. coli cloning vector; αlacZ Apr | Stratagene |
| pTOPO | 3.9-kb pCR 2.1 TOPO cloning vector; Apr | Invitrogen |
| pNZ44 | E. coli-L. lactis vector containing P44 promoter; Cmr | 42 |
| pLPV111 | 4.2 kb; E. coli-L. plantarum/L. sakei shuttle vector; Emr | 6 |
| pSAK20 | 11.8 kb; contains the operon RIRorf4sapKRTE, necessary for transcriptional activation and processing and transport of sakacin A; Cmr | 6 |
| pLPT5 | pLPV111 sakIT | This study |
| pLPT6 | pLPV111 sakTβsakIT | This study |
| pLPT7 | pLPV111 sakTαsakTβsakIT | This study |
| pLPT8 | pLPV111 sakTαsakIT | This study |
| pLPX14 | pLPV111 sakX sakIX | This study |
| pLPXi23 | pLPV111 sakIX | This study |
| pZ235T | pNZ44 sakIT | This study |
| pZ233X | pNZ44 sakIX | This study |
| pSKΔTE | pSAK20 derivative with a 3.5-kb BamHI deletion; sapK+R+ΔsapTE | This study |
| pSK5T | pSKΔTE stxT | This study |
a
LMG, Laboratory of Microbial Gene Technology, Ås, Norway.