TABLE 1.

Summary of purification of α-l-arabinopyranosidase and α-l-arabinofuranosidase from B. breve K-110

Enzyme or purification step	Total activity (μmol/min)	Total protein (mg)	Sp act (μmol/min/mg)
α-l-Arabinopyranosidase
Crude extract	14.30	98.60	0.15
Ammonium sulfate precipitation	12.10	82.83	0.15
DEAE-cellulose column chromatography	8.94	40.14	0.22
Butyl Toyopearl column chromatography	7.20	4.69	1.54
Hydroxyapatite Ultrogel column chromatography	5.61	1.27	4.42
QAE-cellulose column chromatography	2.12	0.39	5.44
Sephacryl S-300 HR column chromatography	0.61	0.07	8.71
α-l-Arabinofuranosidase
Crude extract	10.09	46.10	0.11
Ammonium sulfate precipitation	8.45	37.88	0.22
DEAE-cellulose column chromatography	6.07	18.79	0.45
Butyl Toyopearl column chromatography	3.09	3.64	0.85
Hydroxyapatite Ultrogel column chromatography	1.85	1.50	1.23
Q-Sepharose column chromatography	1.45	0.41	3.54
Sephacryl S-300 column chromatography	0.71	0.11	6.46