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. 2011 Mar 22;286(21):18383–18396. doi: 10.1074/jbc.M111.241182

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Lower level of ATP citrate lyase protein in human islets than in rat islets. Top panel, immunoblot with 15 μg of whole-cell protein in each lane. The actin bands show equal loading of protein across the lanes. Lower panel, immunoblot with 15 μg of whole-cell protein/lane in 1st to 6th lanes. 7th to 9th lanes (from left) had 12, 11, and 9 μg of protein/lane, respectively. The relative densities of each ATP citrate lyase (ATPCL) band are shown at the bottom of the band without correction for the lower amounts of protein in the 7th to 9th lanes that contained rat islet protein.