FIGURE 2.

Growth of yeast strains expressing PMA2 mutants. A, YAK2 yeast strains expressing yeast PMA1 and the indicated PMA2 isoform were grown overnight in dropout galactose medium and then were diluted to an A₆₀₀ of 1, 0.1, or 0.01 and plated on solid YD medium at pH 6.5, 5.5, or 4.5 and allowed to grow for 3 days at 30 °C. B, same as in A, but strains were plated on solid 5′-fluoroorotic acid (5′-FOA) medium at pH 6.5 and allowed to grow for 10 days at 30 °C.