FIGURE 5.

Limited tryptic digestion of wild-type and mutated PMA2. Purified plasma membranes from YAK2 strains (after 5′-fluoroorotic acid selection) expressing wild-type or mutated PMA2 were digested by trypsin as described under “Experimental Procedures” for the indicated times. A, immunodetection of PMA2 using an antibody against the whole protein, allowing the detection of both the full-length enzyme (100 kDa) and the 90-kDa degradation product. B, immunodetection of PMA2 with an antibody against the C-terminal part, detecting only the full-length enzyme (100 kDa).