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. 2011 Mar 31;286(21):18526–18537. doi: 10.1074/jbc.M110.164798

FIGURE 8.

FIGURE 8.

Liposome-embedded GD3 enhanced kinase activity of Yes from GD3− cells and liposome-embedded GM1 suppressed kinase activity of Yes from GD3+ cells. A, in vitro kinase assays using immunoprecipitated Yes from GD3− cells were performed in the presence of liposome-embedded GD3 (5, 10, and 20 μm). C, the relative intensities of phosphorylation bands of enolase and p125 as well as of Yes were measured using National Institutes of Health Image 1.61 and plotted in graphs after correction with that of Yes. The experiments were performed in triplicates, and mean ± S.D. are presented. *, p < 0.05; **, p < 0.01. B, in vitro kinase assays using immunoprecipitated Yes from GD3+ cells were performed in the presence of liposome-embedded GM1 (5, 10, and 20 μm). D, the relative intensities of phosphorylation bands of enolase and p125 as well as Yes were measured using National Institutes of Health Image 1.61 and plotted in graphs as described in C. The experiments were performed in triplicates, and mean ± S.D. are presented. *, p < 0.05; **, p < 0.01. E, in vitro kinase assays using immunoprecipitated Yes from GD3− cells were performed in the presence of liposome-embedded GD3 (1, 2.5, and 5 μm). G, the relative intensities of phosphorylation bands of enolase and p125 as well as of Yes were measured using National Institutes of Health Image 1.61 and plotted in graphs as described in C. The experiments were performed in triplicates, and mean ± S.D. are presented. *, p < 0.05; **, p < 0.01. F, in vitro kinase assays using immunoprecipitated Yes from GD3+ cells were performed in the presence of liposome-embedded GM1 (1, 2.5, and 5 μm). H, the relative intensities of phosphorylation bands of enolase and p125 as well as Yes were measured using National Institutes of Health Image 1.61 and plotted in graphs as described in C. The experiments were performed in triplicates, and mean ± S.D. are presented. *, p < 0.05; **, p < 0.01. Note that significant suppression of kinase activity of Yes derived from GD3+ cells by liposome-embedded GM1 was observed at around 5 μm.