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MCP-1 mRNA and protein levels are reduced in the albino abca4−/− (KO) mice. A, relative MCP-1 mRNA levels by qRT-PCR from 4-week-old BALB/c (WT) and KO mice are shown; each mRNA level was normalized to 18 S rRNA (n = 9; *, p < 0.0005). Ba, a histogram shows the MCP-1 protein levels after normalization to GAPDH (n = 4; *, p = 0.00025). b, shown are representative immunoblots for MCP-1 and GAPDH antibodies using 20 μg of mouse RPE homogenate per lane.
