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. 2011 Apr 4;286(21):18633–18640. doi: 10.1074/jbc.M111.220756

FIGURE 2.

FIGURE 2.

Inhibitor kinetic analysis of SK1 in HEK 293 cells. A and B, V versus S nonlinear regression analysis of stably expressed recombinant SK1 in HEK 293 cells for FTY720 (A) and (S)-FTY720 vinylphosphonate (B). C, effect of varied concentrations of (S)-FTY720 vinylphosphonate on stably expressed recombinant SK1 activity in HEK293 cells assayed with 10 μm sphingosine. D, effect of Bdp-So (50 μm final concentration and varied concentrations, right) and (S)-FTY720 regioisomer (50 μm final concentration) on purified SK1 activity assayed with and without 10 μm sphingosine (left). E, V versus S nonlinear regression analysis for the effect of SKi on recombinant SK1 stably expressed in HEK 293 cells. Results are representative of three independent experiments.