FIGURE 3.

Identification of a core structure of TDP-43^FL fibrillar aggregates. A, peptides corresponding to the mass peaks detected (see supplemental Fig. S1) are mapped on the primary sequence of TDP-43. Details are also summarized in supplemental Table S1. Numbers indicated above the primary sequence of TDP-43 (shown as open bars) represent the amino acid numbers. Four TDP-43 truncates examined here (TDP-43^N, TDP-43^N-1, TDP-43^C, and TDP-43^2-C) are also indicated in the figure. B, an SDS-PAGE analysis of reduced solubility of TDP-43 truncates after agitation with 1,200 rpm at 37 °C for 21 h. Samples were ultracentrifuged at 110,000 × g for 30 min to separate soluble supernatant (s) and insoluble pellets (p). Insoluble pellets were redissolved in PBS containing 2% SDS before loading an SDS-PAGE gel.