Smad2 expression in the developing cerebellar cortex. A–C, sagittal cerebellar sections from E18.5, P7, and P14 mice were subjected to immunohistochemistry using an antibody that specifically recognizes Smad2. Smad2 is expressed in the cerebellum. D, expression of Smad2 in Purkinje cell bodies and external granule cells in P7 mice cerebella. E, Purkinje cell bodies express low levels of Smad2 in P14 mice cerebella. d and e, magnified panels of the area demarcated by a black box are shown in D and E. Cbr, cerebrum; cbl, cerebellum. Scale bar, 50 μm.