FIGURE 2.
NMR spectra of RcsF-Δ30 oxidized monomeric form. A, representative area of the RcsF-Δ30 [15N,1H]HSQC-TROSY spectrum. The backbone HN resonances (generally red, HN resonances of cysteines, purple) are almost completely assigned. The non-assigned black contours in the upper right corner belong to Asn/Gln side chains. B, HNCACB strips demonstrating oxidation of all four Δ30 RcsF cysteines. The HN resonance of Cys-74 is not seen in the RcsF-Δ30 TROSY-HSQC spectrum. The positive Cα resonances are shown in red and the negative Cβ resonances in green. Values of >40 ppm for cysteine Cβ resonances indicate an oxidized state.