FIGURE 4.

Structural similarity of the RcsF-Δ30 oxidized monomeric form to DNA/RNA binding proteins and possible interaction with RNA. A, superimposition of the free L22 protein (cyan, PDB code 1I4J) and RcsF-Δ30 (green). The superimposition with L22 of the Thermus thermophilus 50 S ribosomal subunit (PDB code 2WRJ) is provided in supplemental Fig. 3. B, representative area of the RcsF-Δ30 [¹⁵N,¹H]HQSC-TROSY spectrum before (red) and after (blue) addition of a 2-fold excess of a 14-mer RNA hairpin (left panel). Right panel, mapping of the observed CSP onto the sequence (top) and the structure (bottom). CSP values below 0.018 ppm (3 × statistical error) are shown in green, those between 0.018–0.036 ppm in yellow, and those with more than 0.036 ppm in red.