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. 2003 Dec;69(12):7073–7082. doi: 10.1128/AEM.69.12.7073-7082.2003

FIG. 2.

FIG. 2.

SDS-PAGE (A) and Western blot (B) analyses of the fermentation supernatants of the transformants producing recombinant Xyn11A using Man5A core-hinge carriers. Lanes: 1, purified Xyn11A (1 μg in panel A and 200 ng in panel B); 2, T. reesei ALKO3620; 3, ALKO4332(pALK1010); 4, ALKO4396(pALK945); 5, ALKO4399(pALK948); 6, ALKO4405(pALK1022); 7, ALKO4402(pALK1021). A total of 2 μl of the undiluted and 2 μl of the 1:100 diluted culture supernatants was applied to the Coomassie blue-stained (A) and Western-blotted (B) SDS-polyacrylamide gels. A rabbit polyclonal antibody synthesized against the native Xyn11A was used to detect the recombinant Xyn11A protein. The positions of the Man5A core-hinge- Xyn11A fusion protein (a), Man5A core (b), and Xyn11A (c) are marked by arrows in panel A.