FIG. 3.

Thermostability of xylanase activity on culture supernatants deriving from strains producing recombinant Xyn11A. The thermostability of the xylanase activity was measured in the shake flask cultivation supernatants of the recombinant Xyn11A-producing T. reesei ALKO3620 transformants carrying the expression cassettes pALK945, pALK948, pALK1021, and pALK1022 and in the supernatant of the N. flexuosa fermentation cultivation. Samples to which BSA was added to a concentration of 100 μg/ml were incubated at pH 7 and 70°C, and the xylanase activity was measured at various time points from 0 to 120 min at pH 7 and 70°C using a reaction time of 5 min.