FIG. 5.

Results from 200-ml shake flask cultivations of T. reesei transformants producing Xyn11A. The strains were grown in parallel flasks for 7 days, and the growth was followed by determining the pH and mycelium dry mass. The masses of the lyophilized mycelia (A), the changes in pH (B), the amounts of the proteins secreted into the culture supernatants (C), and the xylanase activities (D) during cultivation are shown. The strains carrying the expression cassettes pALK1284 and pALK1285 were not included in the 200-ml cultivations. The values from duplicate flasks did not differ from each other on average by more than 2.2% (pH), 6.3% (biomass concentration), 6.7% (protein), and 7.0% (xylanase).