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. 2003 Dec;69(12):7073–7082. doi: 10.1128/AEM.69.12.7073-7082.2003

FIG. 7.

FIG. 7.

Western blot analysis of the fermentation supernatants of T. reesei transformants producing Xyn11A. Samples (3 μl of 1:100 dilutions of the culture supernatants) were run in SDS-PAGE, and the recombinant Xyn11A was detected from the Western blot filter by using a polyclonal antibody synthesized against the native Xyn11A. Lanes 1 and 2, T. reesei host strains ALKO3620 and ALKO4468; lanes 3 to 9, T. reesei transformed with the expression cassettes pALK1118, pALK1283, pALK1284, pALK1285, pALK1286, pALK1264, and pALK1022 (the same strains as in Fig. 6); lane 10 contains a 200-ng sample of the purified native Xyn11A protein.