FIG. 8.

Northern blot results for fusion mRNAs expressed from the cel7A promoter. Samples of total RNA (2 μg), isolated from mycelia grown in shake flask cultivations for 3 and 4 days, were loaded in all lanes. The signals obtained by using a ³²P-labeled oligonucleotide hybridizing to the untranslated region of the cel7A promoter were normalized using an actin probe. (A) Relative levels of mRNAs transcribed from the cel7A promoter. The mRNA levels shown are related to the level of the cel7A mRNA from T. reesei ALKO3620 mycelium analyzed on day 4, adjusted to 100. The bars indicate the averages from two parallel cultivations. The variation between the signals analyzed from the samples is shown by the vertical lines. (B and C) Northern blot filters of mycelia grown for 4 days and probed with the ³²P-labeled oligonucleotide probe (B) and with the actin probe (C). The samples in the two lanes of each set of mycelia are from the two separate shake flask cultivations.