TABLE 1.

Production of recombinant Xyn11A protein from strains in which the Man5A core-hinge was used as a carrier for Xyn11A^a

Strain	Expression cassette	Protein (mg/ml)	Xylanase (nkat/ml)	Mannanase (nkat/ml)	Xyn11A (mg/ml)b	Man5A (mg/ml)c	Xyn11A (nmol/ml)d	Man5A (nmol/ml)e
ALKO3620	None	15.3	150	310	ND	0.19	0	0.4
ALKO4332	pALK1010	11.7	NDg	7,800	ND	3.69	0	97.5
ALKO4396	pALK945	10.8	8,650	4,310	0.56	2.04	16.9	49.4
ALKO4399	pALK948	10.9	8,390	3,980	0.54	1.88	16.4	44.9
ALKO4402	pALK1021	10.0	8,610	4,020	0.55f	1.90f	16.8f	46.1f
ALKO4405	pALK1022	10.2	9,150	4,150	0.59	1.96	17.9	46.8

^aThe results are from one fermentation each.

^bThe specific activity determined for purified Xyn11A, 15,600 nkat/mg (Mäntylä and Paloheimo, unpublished), was used in the calculations.

^cThe following specific activities were used: 1,645 nkat/mg for Man5A(ALKO3620) and the average of the two pI forms of purified mannanase (39) and 2,120 nkat/mg for the Man5A core and Man5A core-hinge (determined for the Man5A core protein) (M. Siika-aho, unpublished data).

^dThe calculated molecular mass of 32.9 kDa was used for Xyn11A.

^eThe following calculated molecular masses were used: Man5A, 44.3 kDa; Man5A core, 37.8 kDa; Man5A core-hinge in pALK945 and -948, 41.2 kDa, and in pALK1021 and -1022, 41.9 kDa.

^fThe values were calculated from the activity values. An exact determination is not possible because of partial degradation of the fusion protein. Also, the activities of the partners in the uncleaved fusions are not known.

^gND, not determined.