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Both the motor proteins and the DNA undergo conformational changes during DNA packaging. A, schematic of FRET between different dyes within the DNA and site of action of gp49. Portal-bound Endo VII resolvase action releases the compression from the Y-DNA substrates. B, resolvase release of trapped Y-DNAs is also correlated with increased distance (∼0.6 nm) between the terminase and portal as shown by changes in FRET between the labeled terminase and portal during packaging.
