FIGURE 5.

Effects of Rett Syndrome-associated missense mutations on the conformational flexibility of the isolated MBD. A, the levels of H/DX at 2.5 × 10¹ s are color-coded as indicated on horizontal bars representing peptides derived from the WT MBD and the MBDs harboring the indicated point mutations. Data from all time points monitored (1 × 10¹, 2.5 × 10¹, 1 × 10², and 2.5 × 10² s) are shown in supplemental Figs. 10–13. The black arrows indicate the representative peptide (amino acids 124–138, compare C and D, below). B, the location on the MBD crystal structure (PDB code 3C2I) of the peptide (amino acids 124–138) highlighted in C and D. The positions of the amino acid side chains in the RTT MBD mutants that were investigated are shown as colored spheres. C, comparison of H/DX for a peptide (amino acids 124–138) from the MBD. D, side-by-side analysis of raw data for the WT MBD and the MBDs harboring the indicated mutations. Labeling is as in Fig. 4C.