FIGURE 4.

KAI1 and NDRG1 are down-regulated in prostate cancer and a combination of two genes expression significantly correlates with patient survival. A, immunohistochemical analysis was performed for NDRG1 and KAI1 with prostate tumor specimens from 19 patients. Representative images for immunohistochemical analysis using anti-NDRG1 (upper panels) and anti-KAI1 (lower panels) antibodies were shown. B, staining intensities of both NDRG1 and KAI1 for the specimens were calculated (on a scale of 0–3, with 3 being the highest) for each group of Gleason grade. C, expression of NDRG1 and KAI1 in tissues from normal, tumor, and distant metastatic sites were examined using the cohort dataset of GSE12034. p values are based on a Mann-Whitney Test. *, p < 0.05; **, p < 0.01; ***, p < 0.001. D, A Kaplan-Meier analysis was done to determine the diagnostic value of the combined expression of NDRG1 and KAI1. The p value was determined by log-rank test. E, a multivariate Cox regression analysis was conducted to assess the contribution of the indicated variables to disease prognosis.