FIGURE 2.
Concentration-dependent effects of FAs that are not COX substrates on AA oxygenation by huPGHS-2. Results are shown as increases in the percentage of the rate of O2 consumption from O2 electrode assays of COX activities of huPGHS-2 with 5 μm AA plus the indicated concentrations of various nonsubstrate FAs relative to 5 μm AA alone. O2 consumption was measured using an O2 electrode as described under “Experimental Procedures.” The results are from a single experiment involving triplicate determinations. The error bars indicate ± S.E.