TABLE 3.

Oxygenation of AA at high huPGHS-2 to AA ratios

[1-¹⁴C]AA (1 μm) was incubated with the indicated concentrations of huPGHS-2 at 37 °C for 15 s; the reactions were stopped, and the radioactive products and unreacted AA were separated by radio-HPLC and quantified as described under “Experimental Procedures.” The results show the percentage of the original [1-¹⁴C]AA remaining.

Reaction components	Unreacted [1-14C]AA remaining
	Experiment 1	Experiment 2	Experiment 3	Average ± S.D.
	%
1 μm [1-14C]AA + 100 nm huPGHS-2	0.80	1.38	0.12	0.77 ± 0.63
1 μm [1-14C]AA + 0.5 μm huPGHS-2	6.27	9.74	7.11	7.7 ± 1.8a
1 μm [1-14C]AA + 1 μm huPGHS-2	12.0	9.85	10.9	10.9 ± 1.1a
1 μm [1-14C]AA + 2 μm huPGHS-2	23.3	30.5	25.6	26.5 ± 3.7a

^a Significant difference versus control was with 100 nm huPGHS-2 in Student's t test (p < 0.005).