FIGURE 1.

Identification of CDX4 as a HoxA10 target gene. A, comparison between the proximal 5′-flanks of the human and murine CDX4 gene identified a number of conserved sequences for Hox binding. The 5′-flanks of the human and murine CDX4 genes were compared for conserved sequences (indicated in gray) and Hox/Pbx DNA-binding consensus sequences. Locations of the ChIP chromatin sequence and the truncations used for reporter assays are indicated. B, the proximal 5′-flank co-immunoprecipitated with HoxA10 from U937 cells. ChIP was performed with U937 cells and an antibody to HoxA10 or preimmune serum (as a negative control). Chromatin was amplified by PCR using primers flanking various sequences in the CDX4 5′-flank. Non-precipitated (input) chromatin was a positive control. IP, immunoprecipitation.