FIG. 2.

Comparison of the degradation of 2,4,6-TCP by different types of 2,4,6-TCP-degrading bacteria. Resting cells (OD₆₆₀ = 1; pyruvate grown and 2,4,6-TCP-induced) of R. eutropha MS1 (a and c), and P. tolaasii MS6 (b and d), chosen as representatives of each bacterial type, were incubated in the presence of 0.5 mM 2,4,6-TCP. Samples were taken at intervals, and the UV spectra of the cell-free fluid were obtained (a and b). Representative profiles from several independent determinations are shown. In addition, the concentration of 2,4,6-TCP in the culture fluid was determined by HPLC (c and d). Each point is the average of two replicates; standard deviations were too low to be depicted. Time points (hours) are indicated.