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. 2003 Dec;69(12):7108–7115. doi: 10.1128/AEM.69.12.7108-7115.2003

FIG. 3.

FIG. 3.

Detection of gene sequences involved in 2,4,6-TCP metabolism by PCR and Southern analysis. (a, c, and e) Gel images showing PCR products obtained with primer pairs for (a) TCP-MO (tcpA). (c) HQDO (tcpC), and (e) MAR (tcpD). (b, d, and f) Southern blot using the strain JMP222 tcpA probe (b), the strain MS1 tcpC probe (d), and the strain JMP222 tcpD probe (f). Total DNA from R. eutropha JMP134(pJP4) (lanes 1) R. eutropha JMP222 (lanes 2), R. eutropha MS1 (lanes 3), Ralstonia sp. strain PZK (lanes 4), S. chilensis S37 (lanes 5), S. paucimobilis MS2 (lanes 6), P. tolaasii MS6 (lanes 7), and P. putida MS7 (lanes 8) was digested with Clal. The left lane in panels a, c, and e shows 1-kb (a) or 100-bp (c and e) DNA standards. Arrows indicate fragment sizes for orientation.