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. Author manuscript; available in PMC: 2012 Jan 13.
Published in final edited form as: J Phys Chem B. 2010 Dec 17;115(1):120–131. doi: 10.1021/jp1069708

Table 2.

Criteria

Criterion Description Quantification
A fluorescence fluctuations must be fast enough λ ≥ 0.1 s−1
B diffusion through the evanescent wave must be fast enough λ ≤ 500 s−1
C magnitude of Gs(τ) is high enough Gs(0) ≥ 0.05
D Gs(τ) is not overwhelmed by Ga(τ) Gs(0)/Ga(0) ≥ 0.1
E surface rebinding is negligible P ≤ 0.05

This table describes the criteria used to determine conditions for which TIR-FCS can measure thermodynamic and kinetic parameters describing the interaction of fluorescent molecules with surface binding sites given that the mechanism is a simple bimolecular reaction as defined in Eq. 2.