TABLE 5.
Characteristic melting temperatures of the microcystin synthetase gene E QRT-PCR amplification products (247 bp) obtained using LightCycler melting curve analysis
| Strain | Tma | n | Nucleotide differencesb
|
|||||
|---|---|---|---|---|---|---|---|---|
|
Microcystis sp. strain
|
Anabaena sp. strain
|
|||||||
| GL 260735 | PCC 7806 | PCC 7941 | 90 | 315 | 202A1 | |||
| Microcystis sp. strains | ||||||||
| GL 260735 | 81.3 (0.2) | 12 | ||||||
| PCC 7806 | 81.5 (0.2) | 15 | 2 | |||||
| PCC 7941 | 81.5 (0.1) | 11 | 2 | 1 | ||||
| Anabaena sp. strains | ||||||||
| 90 | 79.7 (0.2) | 12 | 45 | 47 | 47 | |||
| 315 | 79.3 (0.4) | 14 | 45 | 47 | 47 | 0 | ||
| 202A1 | 79.7 (0.2) | 12 | 46 | 48 | 48 | 1 | 1 | |
a
Melting temperature (Tm) is given in degrees and in Celsius. The values in parentheses are CVs and are percentages.
b
Nucleotide differences were calculated for the 209-bp sequence between the primer annealing sites.