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. 2003 Dec;69(12):7044–7057. doi: 10.1128/AEM.69.12.7044-7057.2003

FIG. 3.

FIG. 3.

Comparison, by using PCR amplification and RT-PCR amplification, of DGGE profiles obtained from SMSS from the Nakabusa (A to C) and Yumata (D) hot springs during the present investigation. (A) Changes in 16S rDNA-based DGGE profiles obtained by PCR amplification; the primer set for the domain Bacteria was used. Lane 1, July 2000; lane 2, November 2000; lane 3, May 2001; lane 4, September 2001; lane 5, November 2001; lane 6, June 2002. (B) Changes in 16S rDNA-based DGGE profiles obtained by RT-PCR amplification; the primer set for the domain Bacteria was used. Lane 7, May 2001; lane 8, September, 2001; lane 9, June 2002. (C) DGGE profile obtained with primers Eub341F-GC and Arch915R by RT-PCR amplification. Lane 10, May 2001. (D) DGGE profiles obtained in August 2001 with a primer set for the domain Bacteria by PCR and RT-PCR amplification. Lanes 11 and 12, PCR; lanes 13 and 14, RT-PCR.